Journal: bioRxiv

Article Title: Htr3a receptors control attenuation of fear responses by modulating the corticolimbic activity and synchronization

doi: 10.64898/2026.03.16.711072

Figure Lengend Snippet: Fear conditioning increases pip-induced local field potential (LFP) in control but not in Htr3a-KO during retrieval. A) Schema of the experimental setup for intracerebral recordings in head-fixed mice in the prelimbic cortex (PrL), infralimbic cortex (IL) and basolateral amygdala (BLA). B) Representative examples (1 mouse per condition) of pip-evoked potentials (1-100 Hz), z-normalized to 500ms pre-pip period, in WT and Htr3a-KO mice. Mean of all CS trains (420 pips, solid lines) ± SEM. C) Average pip-evoked LFP ± SEM across the 30 pips of each CS train in WT mice (n = 8; LFP data of 4/12 WT mice were excluded due to poor signal quality) in the PrL, IL, and BLA during habituation and retrieval days. Data are shown across the 14 CS trains (labelled t1 to t14) and were z-normalized to the 500 ms pre-pip baseline. In the PrL, there was a main effect of Days (p = 0.049) and of CS train (p = 0.002). In the IL, there was a main effect of days (p = 0.04), whereas the effect of CS train was not significant (p = 0.77). In the BLA, there was a main effect of days (p = 0.009) and of CS train (p < 0.001). Two-way ANOVA followed by Bonferroni post-hoc tests (*p < 0.05, **p < 0.01, ***p < 0.001). D) Pip-evoked responses in the Htr3a-KO mice (n = 8), no main effects were detected. E) Averaged LFP response of the first 3 CS during habituation and retrieval days, z-normalized to the 500ms pre-pip-onset in WT (main effect of Days: p =0.004, Bonferroni multiple comparison for PrL, IL and BLA: all p < 0.001) and Htr3a-KO (main effect of Days: p = 0.201). F) LFP responses of the first 3 CS in all mice during habituation (main effect of genotype: p = 0.275) and during retrieval (main effect of genotype: p = 0.038, Bonferroni multiple comparison for PrL: p = 0.489, IL: p = 0.097, BLA: *p = 0.043).

Article Snippet: On D5, mice underwent fear conditioning in a sound-attenuating conditioning chamber (17 × 17 × 25 cm; Ugo Basile, 46003), equipped with a grid floor of stainless-steel bars for shock delivery.

Techniques: Control, Comparison

Journal: eLife

Article Title: Gut microbe-derived trimethylamine shapes circadian rhythms through the host receptor TAAR5

doi: 10.7554/eLife.107037

Figure Lengend Snippet: Male or female wild-type mice ( Taar5 +/+ ) or mice lacking the TMA receptor ( Taar5 -/ ) were subjected to a battery of behavioral tests related to cognition, depression, and anxiety including: ( A ) cued fear conditioning, ( B ) elevated plus maze, ( C ) Y-maze, and ( D ) open field test as described in the Methods section. Data are shown for the entire cohort combining both sexes or divided into either male or female cohorts to examine sexual dimorphism in phenotype. Data represent the mean ± SEM from n = 9–10 per group when male and female are separated ( n = 19–20 when both sexes are combined). Significant differences between Taar5 +/+ and Taar5 -/- mice were determined by Student’s t -tests (*p < 0.05).

Article Snippet: Other , Fear Conditioning Chambers , Med Associates , #VFC-008 , Behavioral testing.

Techniques: Battery